ISSN 2167-0404
International Journal of Medicine and Medical Sciences ISSN: 2167-0404, Vol. 2(1), pp. 053-058, 24 January, 2012. © International Scholars Journals
Full Length Research Paper
Cytotoxic Effects of Zinc Oxide Nanoflakes (ZNO NFS) in Human Muscle Carcinoma
Syed M. Usman Ali 1, 4, M. Fakhar-e-Alam 1, 2, Z.Wazir2,M. Kashif3, M. Atif 2, Magnus Willander 1 and W. A. Syed2
1Physical Electronics and Nanotechnology Division, Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174 Norrköping, Sweden
2Department of Physics, Faculty of Basic and Applied Sciences, International Islamic University, Islamabad
3Nano Biochip Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia.
4Department of Electronic Engineering, NED University of Engineering and Technology, Karachi-75270, Pakistan
*Corresponding Author’s E-mail: [email protected]
Received October 29, 2011; Accepted January 24, 2012
Abstract
Cytotoxicity of bare and conjugation of Tween-80 capsulated nanomaterials e.g. zinc oxide nanoflakes (ZnO NFs), iron oxide nanoparticles (Fe2O3 NPs) was examined in dark as well as under light exposure in cell model of immature human muscle carcinoma (RD) via microinjection and free standing drug delivery system. ZnO NFs were grown on the tip of a capillary and characterized by applying atomic force microscopy (AFM) technique and the tip was used as pointer to insert chemicals into cell to visualize/assess the emission of reactive oxygen species (ROS) especially from mitochondria. Bare and conjugated ZnO NFs with δ-aminolevulinic acid (ALA) were irradiated /excited with UV light after cellular uptake, reactive oxygen species were generated. We deduct that ROS is damaging mitochondria resulting in cell necrosis within few minutes. ZnO NFs are not biosafe and have significant toxic effects for both normal as well as cancer cell especially for rhybdomyosarcoma cell line (RD).
Keywords: atomic force microscopy (AFM), δ-aminolevulinic acid (ALA), reactive oxygen species (ROS), human muscle carcinoma (RD cells), Zinc Oxide nanoflakes ZnO NFs.