African Journal of Dentistry

African Journal of Dentistry Vol. 1 (2), pp. 014-018, November, 2013. © International Scholars Journals

Full Length Research Paper

Gender determination using primary teeth: A polymerase chain reaction (PCR) study

Prashant M. Battepati¹ and M. Shodan^2*

¹Department of Pedodontics and Preventive Dentistry, SDM College of Dental Sciences and Hospital, Sattur, Dharwad-

580009, Karnataka, India.

²Department of Public Health Dentistry, SDM College of Dental Sciences and Hospital, Sattur,

Dharwad-580009, Karnataka, India.

*Corresponding author. E-mail: [email protected]. Tel: 09886811031.

Accepted 6 September, 2013

Abstract

The aim of this study was to assess the effect of various environmental factors on the preservation of pulp tissue in primary teeth as a source of DNA and its use for sex determination using polymerase chain reaction (PCR). 120 samples were grouped into 4 groups of 30 teeth each. Group I samples were kept immersed in a bucket of drainage water, group II, III and IV samples were buried in sand taken from seashore, burial ground and desert, respectively for a period of 2 months. Pulp tissue was collected from each sample and DNA was isolated. PCR amplification was performed and sex determination was done by detection of X and Y chromosome-specific alphoid centromeric repeat sequences. In group I, 86% of the samples exhibited correct gender interpretation by PCR amplification. In groups II, III, and IV, all the samples showed correct results indicating a significant difference in scores between group I and the remaining three groups. Teeth stored in dry conditions can serve as a better source of DNA as compared to the teeth stored in moist conditions and co-amplification of both X and Y specific sequences by PCR is a fast, specific, sensitive and reliable method providing sex determination

Key words: DNA, gender, polymerase chain reaction (PCR), forensics