ISSN 2326-7275
International Journal of Anatomy and Physiology ISSN: 2326-7275 Vol. 8 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
The effect of fenugreek on the bioavailability of glibenclamide in normal beagle dogs
Mohamed Fahad Al-Ajmi
Department of Pharmacy, College of Health Sciences, King Saud University, Saudi Arabia. E-mail: [email protected].
Accepted 21 October, 2018
Abstract
Fenugreek (Trigonella foenum-graceum) is an herbal medicine widely used in the traditional medicine to alleviate many diseases including diabetes. Many studies proved its efficacy in reducing blood sugar in diabetic patients. Concurrent administration of fenugreek and glibenclamide may affect bioavailability of glibenclamide. For this reason this study was designed to clarify effect of fenugreek ingestion on bioavailability of glibenclamide in beagle dogs. 4 beagle dogs were administered either glibenclamide alone or glibenclamide with fenugreek and bioavailability of glibenclamide in each of these groups was estimated utilizing HPLC-fluorescence detector method. The method was validated and the results were compared by paired t-test. The method of analysis was linear in the range from 5 to 400 ng/ml and possessed highly specificity and high intra and inter-day precision {1.80 to 9.16% and 5.82 to 10.40% respectively as C.V(%)}. Recovery of glibenclamide (relative to warfarin as IS) was 98.7 to 105.1%. Fenugreek ingestion increased bioavailability of glibenclamide significantly (p< 0.05) compared to control group. The exact mechanism of increased bioavailability of glibenclamide was not studied although literature review of fenugreek constituents points to possibility of increased absorption and/or displacement of glibenclamide from protein binding sites.
Key words: Fenugreek, glibenclamide, Beagle dogs, HPLC, bioavailability.