ISSN 2169-303X
International Journal of Medicinal Plant Research ISSN: 2169-303X Vol. 2 (4), pp. 187-189, April, 2013. © International Scholars Journals
Full Length Research Paper
Effect of growth regulators on the in vitro multiplication of Viola odorata
Zahoor. A. Kaloo*, Rukhsana Akhtar, Zafrul-Haq and B. A. Wafai
Department of Botany University of Kashmir, India.
*Corresponding author. E-mail: [email protected]
Accepted 23 September, 2012
Abstract
The present work as undertaken to study the effect of auxins and Cytokinins on the in vitro multiplication of nodal cuttings with axillary buds and runner tips with apical buds of Viola odorata a medicinal herb growing in Kashmir and western Himalayas. The growth medium used was MS (1962) basal medium supplemented with BAP, Kn and NAA at varying concentrations. The multiplication rate of shoots increased with increasing the concentration of BAP and Kn. However the optimum results were obtained on MS medium supplemented with a combination of 15 µm NAA and 10 µm BAP. Nodal segments showed better results than runner tips.
Key words: Nodal segment, benzylaminopurine, naptheleaciticacid, kinetin, multiplication, viola odorata.