ISSN 2326-7283
African Journal of Internal Medicine ISSN: 2326-7283 Vol. 3 (5), pp. 162-167, June, 2015. © International Scholars Journals
Full Length Research Paper
The therapy of burn wound healing in Rat (Wistar) by using the combination of peripheral blood mononuclear cells (PBMCs) and bone marrow BM-derived mesenchymal stem cell
*1Gusti Revilla, 2Eryati Darwin, 1Yanwirasti and 3Fedik A. Rantam
1Department of Anatomy Faculty of Medicine, Andalas University, Padang, Indonesia.
2Department of Histology, Faculty of Medicine, Andalas University, Padang, Indonesia.
3Stem cell Laboratory Institute of Tropical Disease (ITD) and Laboratory Virology and immunology, Department of Microbiology, Vet. Medicine Faculty Airlangga University Surabaya, Indonesia.
*Corresponding Authors. E-mail: [email protected]. Tel. 062 0751 31746, Fax. 062 0751 32838
Abstract
The therapy to heal the burn wound is still imperfect, therefore it is important to conduct specific research concerning this topic that benefits to society. Using both Bone Marrow-stem cell (BM-MSc) and peripheral blood mononuclear cells (PBMCs) from allogenic donors as part of the therapy to heal the burn wound seems to give positive prospect for the future treatment. In this experiment, PBMC and rat BM- derived from mesenchymal stem cell were used as the therapy model. Immunocytochemistry was used as the method to characterize the phenotype of MSc, it was also used to express the collagen type I and the indirect ELISA in analyzing the TGF-β 1 secretion. The rats with burn wound were divided into 2 kinds of group; the first group of rats was selected to control the use of PBS; while second group of rats was used as the treatment object that was medicated by the applying of the combination of both BM-MSC and PBMC. Stem-cells subcutaneously administered dose applied to each rat was around of 2 x 106 cells. The result showed that the levels of TGF-β 1 secretion in day 3rd and day 7th on the rats which were treated by using the combination of BM-MSC and PBMC were higher compared to the rats from the control group. The experiment that concerns on the thickness of the collagen showed that combination between BM-MSC and PBMC stem cell make it possible in increasing the thickness of collagen I, besides; it also showed significant differences (p=0.000). This research proved that the combination of BM-MSC and PBMC stem cell served can accelerate the healing process for the burn wound on rats through increasing of TGF-β 1 secretion and collagen type 1 expression, it means that PBMCs can be applied as good as chemoattractant.
Key Words: Wound healing, stem cell, allogenic, TGF-β 1, type I collagen.