International Journal of Nematology and Entomology

International Journal of Nematology and Entomology Vol. 1 (6), pp. 121-129, December, 2013. © International Scholars Journals

Full Length Research Paper

Study of using the bacterium Bacillus thuringiernsis israelensis in microbial control of Musca domestica vicina, Diptera Muscidae (Muscidae, Diptera)

Najlaa, Y. Abozinadah*, Faten, F. Abuldahb, Nawal and S. Al-Haiqi

Department of Biology, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.

*Corresponding author. E-mail: [email protected].

Accepted 28 November, 2013

Abstract

The  second  instar  larvae  of  Musca  domestica  were  treated  with  Bacillus  thuringiensis  israelensis, (B.t.i.) at concentrations of 0.5, 1.0, 1.5 and 2.0% under laboratory conditions. Cumulative mortality percentage increased gradually with the increase in B.t.i concentrations and this is represented by straight regression lines indicating homogenity. The LC₃₀ and LC₅₀ were 0.87 and 1.305%, respectively. The tested concentrations indicate significant prolongation in larval duration compared to control. There was an inverse relation between the concentration and pupation percentage and an increase in pupal duration. An insignificant decrease in percentage of adult emergence was observed. Sex ratio was not affected. 2.0% Concentration of B.t.i. caused the greatest reduction in female fecundity (964.5 eggs/female), and the hatchability of eggs also decreased. The pupae and adults appeared malformed when treated with B.t.i. as a 2^nd larval instar.

Key words: Housefly, larvicide, bioassay, mortality, LC₃₀, fecundity, fertility, malformation