ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN: 2169-303X Vol. 5 (4), pp. 262-267, April, 2016. © International Scholars Journals
Full Length Research Paper
Identification of phytochemical constituents of L. nodiflora using TLC and chemical derivatization method
A. P. Abdul1, T. O. Ranvid2 and Anikesh V. Sindura2*
1Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat, India.
2Shree Bapalal Vaidhya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University,
Surat, Gujarat, India.
E-mail: [email protected]
Accepted 30 March, 2016
Abstract
There are a numbers of bioactive compounds in plants, such as alkaloids, tannins, flavonoids, sterols, triterpenes, etc., noted to have the major role in nutrition, physiology and control of diseases. Triterpenoids constitute one of the most characteristic classes of compounds in higher plants. The foremost important task in this paradigm is the screening of these compounds in the plants. The chromatographic study of the compounds serves to be a very useful and reliable source in the process of bioactive compounds screening in plants. According to the ethnobotanical information, it has been reported that the plant Lippia nodiflora possesses the antihypertensive potential. Hence in the present study, an attempt has been made to identify the phytochemical constituents of L. nodiflora using TLC and chemical derivatization method. Further, the isolation of the same compound is carried out by preparative HPTLC using the standardized solvent system viz., hexane: toluene: ethyl acetate (2:15:0.5). The confirmation of the isolation was done by GC-MS.
Key words: Preparative HPTLC, isolation of triterpenoids, Lippia nodiflora, solvent system for triterpenoids separation.