ISSN 2326-7275
International Journal of Anatomy and Physiology ISSN: 2326-7275 Vol. 8 (8), pp. 001-007, August, 2019. © International Scholars Journals
Full Length Research Paper
Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells
Ali Al Shawi1#, Azhar Rasul1#, Muhammad Khan1, Furhan Iqbal2 and Ma Tonghui1*
1Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China.
2Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan
Accepted 14 April, 2019
Abstract
Eupatilin is a flavonoid compound isolated from the Artemisia plant. Eupitillin possesses antioxidant as well as potent anticancer properties. In this study, for the first time, we examined the anti-proliferative effects of Eupatilin in human melanoma A375 cells and its ability to induce apoptosis and cell cycle arrest. Eupatilin specifically induced morphological changes in A375 cells and was less toxic to the normal mouse splenocytes. The inhibitory effects of A375 cells were associated with the DNA damage, apoptosis, and cell cycle arrest at G2/M phase in a dose-dependent manner. The apoptotic effects of Eupatilin were further verified by using Annexin V-FITC/propidium iodide staining in flow cytometry. These results suggest that Eupatilin is an effective natural compound that worth further mechanistic and therapeutic studies against human melanoma.
Key words: Eupatilin, melanoma, apoptosis, cell cycle arrest.