African Journal of Virology Research

ISSN 2756-3413

African Journal of Virology Research ISSN 2756-3413 Vol. 16 (6), pp. 001-007, June, 2022. © International Scholars Journals

Full Length Research Paper

Species composition and molecular analysis of symbiotic fungi in roots of Changnienia amoena (Orchidaceae)

Weimei Jiang1, Guanmei Yang2, Chulong Zhang2* and Chenxin Fu1

1Lab of Systematic and Evolutionary Botany and Biodiversity, College of life sciences, Zhejiang University, Hangzhou 310058, China.

2Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of biotechnology, Zhejiang University, Hangzhou 310058, China.

Accepted 18 February, 2021

Abstract

The diversity and population structure of symbiotic fungi in Changnienia amoena (Orchidaceae) were carried out. A total of 67 isolates were recovered from root samples, all isolates were identified based on morphological criteria and rDNA ITS phylogeny. Overall, 18 taxa were detected. Ascomycetes were the major fungal group, the remaining three isolates were genetically close to Mortierella (Zygomycetes) and Tulasnella (Basidiomycetes), respectively. However, taxa composition of four regions showed only little overlap. These findings provided additional evidence that the non -mycorrhizal endophytes constituted an important component of fungal community in orchids. The potential roles of some species to host were discussed.

Key words: Endophytic fungi, population structure, molecular phylogeny.