ISSN 2756-3790
International Journal of Horticulture and Floriculture Vol. 2 (8), pp. 119-123, August, 2014. © International Scholars Journals
Full Length Research Paper
Vitro seedling development and Asymbiotic seed germination of Epidendrum ibaguense Kunth. (Orchidaceae)
Hasnain Adnan
Institute of Horticultural Sciences, University of Agriculture Faisalabad, Pakistan. E-mail: [email protected].
Accepted 28 July, 2014
Abstract
Effects of four asymbiotic media; Murashige and Skoog (MS), Phytamax (Sigma, USA; PM), (M) and Knudson ‘C’ (KC) were evaluated for seed germination and early protocorm development in Epidendrum ibaguense Kunth. Besides, effects of peptone, activated charcoal and plant growth regulators (6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D)) were also studied. Maximum seed germination was achieved on M and PM (~90%) fortified with 2 gl-1 peptone. Medium supplemented with 1.0 mgl-1 BAP improved seed germination in contrast to basal media whereas 2,4-D either singly or in combination with BAP remarkably reduced the germination rate. Activated charcoal in the medium enhanced germination of seed, induced significantly large size protocorms (1.63±18) and early rooting in protocorms. Within two weeks of culture, spherules emerged out due to cracking of the seed coat. The spherules developed into protocorms with a leaf primordium at apical portion after 3 to 4 weeks and gradually produced complete seedlings. Strong and stout root system was induced in in vitro seedlings on transferring in half strength PM and M media fortified with 0.5-1.0 mgl-1 indole acetic acid (IAA). Well-rooted seedlings were transferred to green house with 90% survival.
Key words: In vitro, seed germination, protocorm, Epidendrum ibaguense, orchid.