ISSN 2375-0715
African Journal of Fisheries Science Vol. 1 (2), pp. 011-015, July, 2013. © International Scholars Journals
Full Length Research Paper
Genetic diversity evaluation of Paraschistura bampurensis (Nikolskii, 1900) in Shapour and Berim Rivers (Iran) using microsatellite markers
Ghasem Askari* and Ali Shabani
Fishery Faculty, Gorgan University of Agriculture Science and Natural Resources. Iran.
*Corresponding author. E-mail: [email protected].
Accepted 4 March, 2013
Abstract
Paraschistura bampurensis is one of the species of genus Paraschistura. It is distributed in Iran and Pakistan freshwater. This paper reports a study carried out on genetic diversity and population structure of P. bampurensis in Shapour and Berim river basins, Iran. 100 samples of 50 fish each were collected from each river and were used. Microsatellite markers have been increasingly used in population genetics studies. In this study, a total of six microsatellite loci and two sampling populations were used. The average number of observed alleles in Shapour and Berim population accounted for 13.5 and 12.5, respectively. The average number of allele’s level in the population accounted for 13, well above the reported values for freshwater fishes. The mean expected and observed heterozygosity mean were 0.562 and 0.861, respectively. Approximately all loci showed a deviation from Hardy-Weinberg equilibrium. The genetic similarity and distance between the two populations were 0.402 and 0.669, respectively. According to the analysis, it seems that P. bampurensis has a desirable genetic diversity in the investigated regions.
Key words: Paraschistura bampurensis, genetic diversity, microsatellite markers, Shapour River, Berim River.