ISSN 2375-0731
African Journal of Pig Farming ISSN 2375-0731 Vol. 7 (1), pp. 001-004, January, 2019. © International Scholars Journals
Review
Review of somatic cell nuclear transfer in pig
Suchitra Muenthaisong1,2, Andras Dinnyes1,2,3 and Tshimangadzo Lucky Nedambale4,5,6*
1Molecular Animal Biotechnology Laboratory, Szent Istvan University, H-2100 Gödöllö, Hungary.
2BioTalentum Ltd, H-2100 Gödöllö, Hungary.
3Dept. of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
4Agricultural Research Council (ARC), Animal Production Institute, Germplasm Conservation and Reproductive Biotechnologies, Irene, 0062, South Africa.
5Tshwane University of Technology, Department of Animal Sciences, Private Bag X680, Pretoria 0001, South Africa.
6University of the Free State, Department of Animal, Wildlife and Grassland Sciences, P.O. Box 339, Bloemfontein, South Africa.
Accepted 30 September, 2018
Abstract
It is now more than 8 years, since the first cloned pig from nuclear transfer was reported. Success of somatic cell nuclear transfer (SCNT) in pig is still low compared to that in bovine. Embryonic and neonatal abnormalities of cloned piglets are probably a result of incorrect or incomplete reprogramming of the transferred donor cell nuclei. Recently, technical refinements have resulted in a considerable increase of SCNT efficiency. The current level of efficiency is already sufficient for special purposes with high scientific and commercial impact including xenotransplantation, generation of human disease models and for biomedical studies. In this paper, we review current pig cloning methodologies for the technical and potential applications.
Key words: Pig, somatic cell, nuclear transfer, oocyte and embryo.