ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 8 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Plant regeneration from leaf explants of Barringtonia racemosa
Mandana Behbahani1, Abdul Manaf Ali2 and Radzali Muse3
1’2Department of Cell and Molecular Biology, Department of Cell and Molecular Biology, UPM, Malaysia
3Department of Biochemistry, Department of Cell and Molecular Biology, UPM, Malaysia
Accepted 22 November, 2018
Abstract
A protocol for in vitro propagation from leaf explants of Barringtonia racemosa is reported. Calli were aseptically raised by placing surface sterilized leaf explants on Woody Plant Medium (WPM) supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D). On the shoot induction medium, the callus induced on the WPM medium containing 2 mg/L (w/v) KIN+0.2 mg/l (w/v) IBA and 2 mg/L (w/v) of KIN +0.4 mg/L(w/v) of NAA was the most effective, providing high shoot regeneration frequency of 85.6 and 76.5% respectively. In addition, the highest number of shoots produced was 8.2 and 6.3 shoots per explant respectively in the medium containing the mentioned phytohormones. The good rooting percentage 62 and 5.6% roots per shoot were achieved on WPM medium supplemented with 3g/L (w/v) of active charcoal and 0.8 mg/L (w/v) of IBA. Approximately 96% of the in vitro raised plantlets with well developed shoots and roots were survived after transferring to mixed soil and grown well in the glass house.
Keywords: Barringtonia racemosa, rooting, shoot regeneration.