ISSN 2756-3340
African Journal of Infectious Diseases Research ISSN 2756-3340 Vol. 8 (5), pp. 001-005, May, 2021. © International Scholars Journals
Full Length Research Paper
Genetic fingerprinting and phylogenetic diversity of Staphylococcus aureus isolates from Nigeria
Onasanya A.1,2*, Mignouna H.D2,α and Thottappilly G.2,٨
1Department of Microbiology, Federal University of Technology Akure, Akure, Nigeria.
2International Institute of Tropical Agriculture, PMB 5320, Ibadan, Nigeria.
Accepted 23 April, 2021
Abstract
Genetic fingerprinting of 18 different isolates of Staphylococcus aureus from Nigeria using random amplified polymorphic DNA (RAPD) was carried out. Ten out of 100 Operon primers showed polymorphism among the isolates tested generating 88 bands, 51 of which were polymorphic with sizes ranging between 200 and 3,000 bp. All the isolates were classified completely into two major groups (Sa-1 and Sa-2) with twelve different subgroups. Sa-1 group originated from human while isolates from plant and animal origins formed the Sa-2 group. The twelve different subgroups suggest adaptation of S. aureus in the different host cells. This indicates possible relationship between host origin and genetic variation among S. aureus isolates. The DNA fingerprint defined for each race of S. aureus could be useful in epidemiological studies, medical diagnosis and the identification of new strains and their origins.
Keywords: Staphylococcus aureus, foodborne-acquired infections, genetic fingerprinting; phylogenetic diversity, RAPD, polymorphism.