International Journal of Diseases and Disorders ISSN 2329-9835 Vol. 5 (1), pp. 001-010, January, 2017. © International Scholars Journals
Full Length Research Paper
Statistical optimization of low-cost medium for economical production of Bacillus subtilis biosurfactant, a biocontrol agent for the olive moth Prays oleae
Dhouha Ghribi1,2*, Ines Mnif1,2, Hanen Boukedi3, Radhouane Kammoun2 and Semia Ellouze-Chaabouni1
1Unité Enzymes et Bioconversion, National School of Engineers of Sfax, Tunisia.
2Higher Institute of Biotechnology of Sfax, Tunisia.
3Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia.
Accepted 11 November, 2016
Abstract
Biosurfactants are currently not a feasible alternative to chemically synthesized surfactants as a result of their potentially high production costs. In this work, Bacillus subtilis SPB1 biosurfactant was shown to be efficient in the biocontrol of the olive moth Prays oleae. Its production was improved by optimizing the medium components using inexpensive substrates. The effect of orange peels, soya bean and diluted sea water on SPB1 biosurfactant production was studied and was adjusted using central composite design. The experimental results were fitted to a second-order polynomial model that yielded a determination coefficient of R2=0.932. The optimal medium for biosurfactant production was found to be composed only by orange peels (15.5 g/L), soya bean (10 g/L) and diluted sea water (30%). The predicted and observed response were 4.3 g/L (with desirability = 0. 21) and 4.45 g/L, respectively. In comparison to original level production, two fold increases had been obtained.
Key words: Bacillus subtilis, biosurfactant, central composite design, optimization, Prays oleae.
Full Length Research Paper
Statistical optimization of low-cost medium for economical production of Bacillus subtilis biosurfactant, a biocontrol agent for the olive moth Prays oleae
Dhouha Ghribi1,2*, Ines Mnif1,2, Hanen Boukedi3, Radhouane Kammoun2 and Semia Ellouze-Chaabouni1
1Unité Enzymes et Bioconversion, National School of Engineers of Sfax, Tunisia.
2Higher Institute of Biotechnology of Sfax, Tunisia.
3Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia.
Accepted 11 November, 2016
Biosurfactants are currently not a feasible alternative to chemically synthesized surfactants as a result of their potentially high production costs. In this work, Bacillus subtilis SPB1 biosurfactant was shown to be efficient in the biocontrol of the olive moth Prays oleae. Its production was improved by optimizing the medium components using inexpensive substrates. The effect of orange peels, soya bean and diluted sea water on SPB1 biosurfactant production was studied and was adjusted using central composite design. The experimental results were fitted to a second-order polynomial model that yielded a determination coefficient of R2=0.932. The optimal medium for biosurfactant production was found to be composed only by orange peels (15.5 g/L), soya bean (10 g/L) and diluted sea water (30%). The predicted and observed response were 4.3 g/L (with desirability = 0. 21) and 4.45 g/L, respectively. In comparison to original level production, two fold increases had been obtained.
Key words: Bacillus subtilis, biosurfactant, central composite design, optimization, Prays oleae.Full Length Research Paper
Statistical optimization of low-cost medium for economical production of Bacillus subtilis biosurfactant, a biocontrol agent for the olive moth Prays oleae
Dhouha Ghribi1,2*, Ines Mnif1,2, Hanen Boukedi3, Radhouane Kammoun2 and Semia Ellouze-Chaabouni1
1Unité Enzymes et Bioconversion, National School of Engineers of Sfax, Tunisia.
2Higher Institute of Biotechnology of Sfax, Tunisia.
3Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia.
Accepted 11 November, 2016
Biosurfactants are currently not a feasible alternative to chemically synthesized surfactants as a result of their potentially high production costs. In this work, Bacillus subtilis SPB1 biosurfactant was shown to be efficient in the biocontrol of the olive moth Prays oleae. Its production was improved by optimizing the medium components using inexpensive substrates. The effect of orange peels, soya bean and diluted sea water on SPB1 biosurfactant production was studied and was adjusted using central composite design. The experimental results were fitted to a second-order polynomial model that yielded a determination coefficient of R2=0.932. The optimal medium for biosurfactant production was found to be composed only by orange peels (15.5 g/L), soya bean (10 g/L) and diluted sea water (30%). The predicted and observed response were 4.3 g/L (with desirability = 0. 21) and 4.45 g/L, respectively. In comparison to original level production, two fold increases had been obtained.
Key words: Bacillus subtilis, biosurfactant, central composite design, optimization, Prays oleae.