African Journal of Fisheries Science ISSN 2375-0715 Vol. 9 (1), pp. 001-006, January, 2021. © International Scholars Journals

Full Length Research Paper 

Effect of cadmium chloride and ascorbic acid exposure on the vital organs of freshwater Cyprinid, Labeo rohita

Abdul Latif1, Muhammad Ali2, Rahila Kaoser3, Rehana Iqbal1, Kashif Umer1, Muhammad Latif1, Shazia Qadir1 and Furhan Iqbal1*

1Institute of Pure and Applied Biology, Zoology Division. Bahauddin Zakariya University Multan 60800, Pakistan.

2Institute of Biotechnology. Bahauddin Zakariya University Multan 60800, Pakistan.

3Department of Chemistry,. Bahauddin Zakariya University Multan 60800, Pakistan.

Accepted 08 October, 2020

Abstract

The present study was carried out to evaluate the impact of sub lethal concentrations of heavy metal, cadmium chloride (CdCl2.H2O), ascorbic acid (vitamin C) and their combination on Labeo rohita. The effect was investigated on the basis of histopathological examinations of control and experimental groups exposed to heavy metal. The acute semi statistical toxicity test for L. rohita revealed 96 h LC50 value 22.92 mg L-1 for cadmium chloride (CdCl2.H2O). 215 fingerlings of L. rohita were exposed to three different experimental conditions for 96 h: 11.46 mg L-1 CdCl2.H2O, or 450 mg Kg -1 ascorbic acid or combination of both these doses. No alterations were observed in gill sections upon comparison between treated and untreated groups. Congestion in sinusoids, fatty change, an increase in Kupffer cells and intrahepatic lymphocytes was observed in CdCl2.H2O treated group. In kidney sections of heavy metal treated group, degeneration of the glomerular tissue, occlusion in tubular lumen and necrosis were observed. Similar changes but in less severe form, as described above, were observed in the fish exposed to combination of CdCl2.H2O and ascorbic indicating that ascorbic acid do detoxify the effect of heavy metal to some extant. Our results indicate that cadmium chloride (CdCl2.H2O), if present in fresh water bodies, may act as strong toxic agent for L. rohita.

Key words: Labeo rohita, cadmium chloride (CdCl2.H2O), ascorbic acid, histopathology.