International Journal of Biochemistry and Biotechnology Vol. 1 (5), pp.162-166, July, 2012. © International Scholars Journals  

Full Length Research Paper

 

Detection of Blactx-M gene on resistant salmonella enterica from a hospital in southeast Nigeria

1Adeleke OE, 1Onyenwe NE*, 2Mbata TI

 

1Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.

2Department of Microbiology, Federal Polytechnic Nekede Owerri, Nigeria.

Corresponding Author’s E-mail: o_nathejik@yahoo.com

Received July 13, 2012; Accepted July 31, 2012

Abstract

Typhoid fever is said to be a systemic infection caused by Salmonella Enterica serotype typhi. A total of fifty stool samples were analyzed out of which twenty-five clinical isolates of Salmonella enterica serovars recovered between July-September, 2010 were investigated and screened using molecular methods for the presence of Blactx-M type gene. Polymerase Chain Reaction analysis showed that the four of the S.enterica encoding the CTX-M type gene were mostly Extended Spectrum β-lactamase producers which had no plasmids DNA. Phenotypic analysis reveals that the enzymes were active on Ceftazidime (64%) more than Cefotaxime (16%). Two folds serial dilution method reveals that Cefotaxime had increased Minimum Inhibitory Concentration (MIC) ranging from 1.56ug/ml- 12.5ug/ml depending on the isolates and the β-lactamase enzyme produced. The four Blactx-M genes detected on the adult patients were from the General Out Patients Department (GOPD) and National Health Insurance Scheme (NHIS) section of the hospital in Eastern Nigeria.

Key words: Blactx-M, ESBL, Salmonela enterica, PCR, Cefotaxime, Ceftazidime.