International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 10 (1), pp. 001-009, January, 2021. © International Scholars Journals

Full Length Research Paper

Effects of organophosphate insecticide, malathion on the liver and kidney of male Wistar albino rats by α-tocopherol and selenium

Abdulaziz M. Al-Othman1, Khaled S. Al-Numair1, Gaber E. El-Desoky2,3, Kareem Yusuf2, Zeid A. Al Othman2, Mourad A. M. Aboul-Soud3,4* and John P. Giesy5,6,7,8

1Department of Community Health Sciences, College of College of Applied Medical Science, King Saud University, Kingdom of Saudi Arabia.

2Department of Chemistry, College of Science, King Saud University, Kingdom of Saudi Arabia.

3Biochemistry Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.

4College of Science, King Saud University, P. O. BOX 2245, Riyadh 11451, Kingdom of Saudi Arabia.

5Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

6Department of Zoology, and Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA.

7School of Biological Sciences, University of Hong Kong, Hong Kong, SAR, China.

8Zoology Department, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.

Accepted 22 September, 2020

Abstract

Protection from effects of the organophosphate insecticide, malathion on the liver and kidney of male Wistar albino rats by -tocopherol and selenium was investigated. Significantly greater (P<0.01) mean concentrations of malondialdehyde (MDA) and lesser concentrations (P<0.01) of reduced glutathione (GSH) and tissues total proteins were observed in liver and kidney of rats exposed to malathion. Activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were less in livers and kidneys of rats exposed to malathion. These effects caused by exposure to malathion were reversed when rats were subsequently treated orally with 100 mg/kg b.w. tocopherol and/or 0.1 mg selenium/kg b.w. Both tocopherol and selenium (Se) reduced the concentration of MDA, and increased concentration of total proteins and reduced glutathione and mean activities of SOD, CAT and GPx, to levels that were not significantly different from unexposed, control rats, previous to exposure to malathion. Selenium was significantly ( P<0.05) more effective at recovering activities of SOD and GPx in liver and kidney of malathion treated rats than was - tocopherol, whereas, -tocopherol was significantly (P<0.05) more effective at recovering activities of CAT in liver and kidney than was Se. Both -tocopherol and Se were effective in alleviating oxidative damage in liver and kidney of rats caused by malathion.

Key words: Oxidative stress, antioxidant enzymes, lipid peroxidation, vitamin E, selenium.