International Journal of Urology and Nephrology

International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (2), pp. 001-008, February, 2018. © International Scholars Journals

Full Length Research paper

Effective inhibition of agglutinability of human A and B type erythrocytes by sodium periodate -chloroacetic acid combined treatment

Dipankar Ghosh1*, Susmita Santra1, Sujit Dutta2 and Panchanan Pramanik1

1Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.

2Hematology and Blood Bank Division, Kharagpur Sub-divisional Hospital 721302, India.

Accepted 14 September 2017

Abstract

The study focused on effective inhibition of the agglutinability of human A and B type erythrocytes by sodium periodate-chloroacetic acid concerted action. Suppression of agglutinability was determined by using anti-A and anti-B antisera. Effective concentrations of sodium periodate were 6.8 and 2.7 mM, respectively for A and B type erythrocytes in presence of 4.5 mg.ml-1 chloroacetic acid in the reaction mixture. Some major bio-physiological consequences of human erythrocytes including agglutination index, spontaneous red blood cell lysis percentage, zetapotential, morphology, surface functional groups (- OH, > C = O, -COOH) and semi- quantitative oxygen uptake and phagocytic uptake were also analyzed after the chemical treatments. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.

Key words: ABH antibodies, agglutination, concerted action, erythrocyte, medicine, transfusion.