International Journal of Pharmacy and Pharmacology

International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 2 (5), pp. 088-093, August, 2013. © International Scholars Journals

Full Length Research Paper

Urinary excretion of mannose and mannose related compounds in humans consuming Aloe saponaria pulp

Claudia S. Fallati 1, María E. Olivera1, 2, Laura C. Luciani-Giacobbe 1, 2, Carolina B. Romañuk1,2, Ruben H. Manzo* 1,2

1Department of Pharmacy, Faculty of Chemical Sciences, National University of Cordoba. Haya de la Torre  and Medina Allende, Ciudad Universitaria (5000), Cordoba, Argentina.

2Unit of Research and Development in Pharmaceutical Technology (UNITEFA)-CONICET, Argentina.

*Corresponding author. Email: rubmanzo@fcq.unc.edu.ar. Tel. 54-351-5353865

Accepted 17 June, 2013

 Abstract

The adhesion of Escherichia coli to uroepithelium can be altered by the interaction between specific carbohydrate molecules and the receptors on the bacterial surface. Mannose is one of the most potent inhibitors among carbohydrates. Aloe saponaria, currently used as a dietary supplement, contains polymannanes as main components. This work was designed to evaluate the mannose oligosaccharide metabolites excretion after oral intake of Aloe saponaria pulp in order to estimate its potential utility in preventing urinary infections. Five volunteers received a daily oral intake of Aloe saponaria pulp for 7 days. Urine samples were collected at time 0 and on the seventh day and assayed for their mannose contents by Dubois method, TLC, HPLC and 1H-NMR. The results showed that the oral intake of Aloe Saponaria fresh pulp produced sugar excretion, composed mainly of mannose and mannose related compounds, suggesting that Aloe saponaria pulp could be a potential therapeutic agent for prevention of urinary tract infection.

Key words: Aloe saponaria, mannose, urinary excretion, urinary tract infection, anti-adhesion therapy, uropathogenic Escherichia coli.