International Journal of Diseases and Disorders

International Journal of Diseases and Disorders ISSN 2329-9835 Vol. 6 (7), pp. 001-008, July, 2018. © International Scholars Journals

Full Length Research Paper

Isolation and identification of cultivable lactic acid bacteria from traditional goat milk cake in Yunnan province of China

Qiuhua Bao, Xia Chen, Hongxia Liu, Wenyi Zhang, Wenjun Liu, Jie Yu, Fang Wang and Heping Zhang*

The Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, 306 Zhaowuda street, Hohhot, China, 010018.

Accepted 12 January, 2018

Abstract

Thirteen goat milk cake samples which were collected from different households in Yunnan province in China were used for isolation and identification of lactic acid bacteria (LAB). The results showed that LAB and yeasts were the dominant microflora in the samples with the LAB counts in all samples ranged from 8.32 log10 CFU/g to 10.16 log10 CFU/g. The number of yeasts in most samples ranged from 1.60 log10 CFU/g to 6.15 log10 CFU/g respectively. Seventy-six strains of LAB were isolated from these samples and identified by conventional and 16S rRNA gene sequence analysis methods. The LAB strains were found to belong to five general and nine different species, among which the ratio of cocci bacteria (96.1%) was higher than that of rod (3.9%), and was considered to be the major population. The results also showed that Lactococcus lactis, subsp. lactis (72.4%) and Lactococcus garvieae (10.5%) could be considered as the dominant groups population in these samples. The obtained LAB pure cultures may serve as valuable sources for further selection of starter cultures in dairy industry.

Key words: Goat milk cake, Lactic acid bacteria, isolation, identification and 16S rRNA gene sequencing analysis.