International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 11 (2), pp. 001-004, February, 2021. © International Scholars Journals

Review

Advances in DNA sequencing: Challenges and limitations of personal sequencing

 ¹K.   Mehla¹, S. Chaudhary², A. Kumar³, V. Kumar³, P. Chauhan⁴, S. Gupta⁵, J. Singh⁶, P. Kumar⁷, V. Kumar⁸, N. Kumar³, A. Jindal³, S.Kumar³, V. Sharma³, S. Chand³, N. Mahajan³, A. Singh³, B. Ramesh⁹and D. Singh³*

¹Institute of Genomics and Integrated Biology, Mall Road, New Delhi, India

²Banasthali Vidyapith, Rajsthan, India

³Molecular Biology Laboratory, Department of Genetics and Plant Breeding, SVP University of Agriculture and Technology, Meerut -250110, India.

⁴Department of Cell Biology, SVP University of Agriculture and Technology, Meerut -250110, India.

⁵Department of Molecular Biology and Genetic Engineering, SVP University of Agriculture and Technology, Meerut -250110, India.

⁶Department of Immunology and Defense Mechanism, SVP University of Agriculture and Technology, Meerut -250110, India.

⁷Department of Physiology and Biochemistry, SVP University of Agriculture and Technology, Meerut -250110, India.

⁸Department of Ag. Biotechnology, AAU, Anand, Gujarat, India.

⁹Department of Genetics and Plant Breeding, CCS University, Meerut, UP, India.

Accepted 04 January, 2021

Abstract

The advent of second and third generation DNA sequencing techniqnologies have revolutionized the genomics research. The Single Molecule Sequencing technologies are adequate to minimize occurrence of errors in sequencing. The third generation sequencing technologies could overcome limitations of first and second-generation sequencing technologies. The almost complete human genome sequencing was performed by using single molecular sequencing technologies. Benefits, risks and safeguards associated with the disclosure of information of very intimate kind contained in human genome, were also discussed. The power of whole sequencing for human welfare arises only from associations with medical histories, behavioral characteristics, physical descriptions and genome-environment interactions not from mere knowledge of base pairs.

Key words: DNA sequencing, medical histories, behavioral characteristics, physical descriptions.