Frontiers of Agriculture and Food Technology

Frontiers of Agriculture and Food Technology ISSN 7295-2849 Vol. 10 (3), pp. 001-004, March, 2020. © International Scholars Journals

Full Length Research Paper

In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (L.) Del.

Mansor Ndoye1, Ismaïla Diallo2 and Yaye Kène Gassama/Dia1

1Laboratoire de biotechnologies, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop Dakar, B.P. 5005, Dakar, Sénégal.

2ISRA-DRPF, Route des Pères Maristes, B.P. 2312, Dakar, Sénégal.

Accepted 18 October, 2019

Abstract

Procedures were developed for micropropagation of Balanites aegyptiaca using axillary bud explants obtained from mature trees. Cultures were established in Murashige and Skoog (MS) medium supplemented with 2.5 mg/l 6-benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA). The effects of kinetin on shoot growth and proliferation in vitro was also investigated. Results show that shoot multiplication required 2.5 mg/l of BAP. Shoot length was significantly affected by the presence of BAP or 6-furfurylaminopurine (Kin). Rooting of shoots in vitro was achieved on MS medium containing 20 mg/l of the auxin, indolebutyric acid (IBA). Rooted shoots acclimated and were successfully transferred into soil, with 48% of the plantlets surviving.

Key words: Balanites aegyptiaca, micropropagation, rooting.