African Journal of Pig Farming

African Journal of Pig Farming ISSN 2375-0731 Vol. 8 (3), pp. 001-007, March, 2020. © International Scholars Journals

Full Length Research Paper

Tetracycline resistance genes in Pasteurella multocida isolates from bovine, ovine, caprine and swine pneumonic lungs originated from different Greek prefectures

Maria Babetsa1,2, Vassilios Sandalakis4,5, Christina Vougidou3, Antonios Zdragas1, Afroditi Sivropoulou2, Anna Psaroulaki4,5 and Loukia V. Ekateriniadou1*

1National Agricultural Research Foundation, N.AG.RE.F., Veterinary Research Institute of Thessaloniki, N.AG.RE.F Campus of Thermi, 57001, Thermi, Thessalonki, Greece.

2Department of Biology, Aristotle University of Thessaloniki, Campus of Thessaloniki 54124, Thessaloniki, Greece. 3Institute of Infectious and Parasitic Diseases of Thessaloniki, Ministry of Rural Development and Food, 54627, Thessaloniki, Greece.

4Regional Laboratory of Public Health of Crete, 71110, Heraklion, Crete, Greece.

5Laboratory of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, WHOcc, University of Crete, 71110, Heraklion, Crete, Greece.

Accepted 13 October, 2019

Abstract

The aim of the present work was to analyze the antibiotic resistance of Pasteurella multocida isolates from sheep, goats, cattle and pigs originated from eight different Greek prefectures, to determine the class(es) of the tet genes, to investigate any possible association with plasmids or/and chromosomes and to study the geographical distribution of the resistant isolates. Eighteen out to one hundred isolates were resistant to tetracycline with a minimal inhibitory concentration (MIC) higher than 128 μg/ml. The tetH gene was found in thirteen (72.2%) and the tetB gene was found in four isolates (22.2%). After treatment with the restriction enzymes EcoRV, EcoRI, PstI and BspHI, the tetB gene showed a new polymorphism. Partial sequencing of the tetH and tetB genes revealed five polymorphisms in the tetB gene and two polymorphisms in the tetH gene; phylogenetic and molecular evolutionary analyses were also conducted. Both tetB and tetH genes revealed patterns clearly differentiated from the rest of Pasteurella species meaning that the genes could be transferred from a non Pasteurella species. The results presented provide further knowledge and evidence of the role played by commensals as of resistance determinants.

Key words: Pasteurella multocida, tet genes, multiple sequence analysis.


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African Journal of Pig Farming

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