African Journal of Pig Farming
African Journal of Pig Farming ISSN 2375-0731 Vol. 8 (3), pp. 001-007, March, 2020. © International Scholars Journals
Full Length Research Paper
Phylogenetic and pathogenetic analysis of Streptococcus suis serotype 7 strain HW07 isolated from diseased pig in China
Shujie Wang1,2, Sen Hu2, Jiamin Jin1,2, Yonggang Liu2, Gang Wang 2, Yabin Tu2, Chenggang Jiang2, Xuehui Cai2* and Xiuying Zhang1*
1Pharmacology Laboratory, Animal Medical College, Northeast Agriculture University, Harbin 150030, China.
2State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, People’s Republic of China.
Accepted 08 December, 2019
A recent isolate of Streptococcus suis serotype 7 (SS7), named HW07, was isolated from brains of pigs suspected with Streptococcus suis (S. suis) infection. The sequence types (ST) of this isolate was determined by Multilocus sequence typing (MLST) method. In order to evaluate pathogenesis of HW07 isolate, zebrafish and pigs were inoculated with HW07 strain by peritoneal cavity and vein. The results demonstrate that the 50% lethal dose (LD50) of HW07 isolate was 1.25 × 105 colony forming units (CFU)/fish and could induce pig disease, the ST number of HW07 isolate is a novel ST-ST335. Molecular and phylogenetic analysis for the recA gene of HW07 isolate showed that it joins to the I group cluster of S. suis strains, the predominant genotype in China.
Key words: Streptococcus suis serotype 7, multilocus sequence typing, the sequence type, phylogenetic analysis.
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