African Journal of Pig Farming

African Journal of Pig Farming ISSN: 2375-0731 Vol. 10 (1), pp. 001-005, January, 2021.  © International Scholars Journals

Full Length Research Paper 

Antiviral activities of ellagitannins against bovine herpesvirus-1, suid alphaherpesvirus-1 and caprine herpesvirus-1

Neli Vilhelmova-Ilieva1, Ivo Sirakov2, Remi Jacquet3, Stephane Quideau3 and Angel S. Galabov1*

1Department of Virology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 G. Bonchev Str., BG-1113 Sofia, Bulgaria.

2Department of Medical Microbiology, Medical Faculty, Medical University of Sofia, 2 Zdrave Street, BG-1431 Sofia, Bulgaria.

3Universitéde Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie,

2 rue Robert Escarpit, 33607 Pessac Cedex, France. *Corresponding author. E-mail: Tel: +359 888 287 103. Fax: +359 2 870 0109.

Accepted 23 July, 2021


In vitro antiviral activity of three ellagitannins - castalagin, vescalagin and grandinin was investigated against replication of three of the most common herpes viruses infecting animals, namely bovine herpesvirus-1 (BoHV-1), suid alphaherpesvirus-1 (SuHV-1), and caprine herpesvirus-1 (CapHV-1). The cytopathic effect inhibition test via the neutral red uptake assay in Madin-Darby bovine kidney (MDBK) cell culture was applied. The highest activity was demonstrated by castalagin followed by vescalagin against replication of SuHV-1, strain A2, with selectivity index values of 336.8 and 309, respectively, which are close to that of acyclovir (SI = 540). The activity of grandinin against the replication of SuHV-1 A2 strain (SI = 40.8) as well as the activity of all three substances against the replication of BoHV-1 strain K22 was lower but still significant (castalagin SI = 45; vescalagin SI = 42.5; grandinin SI = 32.3). Against the E/CH strain of CapHV-1, the three ellagitannins showed a moderate to weak activity: Castalagin SI = 19.3, vescalagin SI = 18.8, grandinin SI = 11.8. The results obtained characterize compounds tested as perspective antivirals.

Key words: bovine herpesvirus-1, suid alphaherpesvirus-1, caprine herpesvirus-1, ellagitannins, alpha acyclovir, antiviral activity.