African Journal of Ecology and Ecosystems

African Journal of Ecology and Ecosystems ISSN: 2756-3367 Vol. 9 (7), pp. 001-006, July, 2022. © International Scholars Journals

Full Length Research Paper

Assessment of the physiological and biochemical characterization of a Lactic acid bacterium isolated from chicken faeces in sahelian region

Ali Bayane1,*, Dominique Roblain1, Robin Dubois Dauphin1, Jacqueline Destain1, Brehima

Diawara2, Philippe Thonart1

1FUSAGx, Unité de Bio-industrie, Centre Wallon de Biologie Industrielle (CWBI). Passage des Déportés 2, 5030 Gembloux, Belgique.

2Département de Technologie Alimentaire, IRSAT/CNRST. 03 BP 7047 Ouagadougou 03 Burkina Faso.

Accepted 12 May, 2022

Abstract

The aim of this work was the examination of the microbiological and technological properties of a lactic acid bacterium (CWBI-B623) isolated from chicken faeces in sahelian region (Burkina Faso). The strain CWBI-B623 is a Gram positive rod, asporogenous, catalase-positive, facultatively anaerobic, non motile and mesophilic. The analysis showed that it produce L(+)-lactic acid via homofermentative pathway and it was able to ferment an important number of the carbohydrates of API 50CHL system. The 16S-rDNA-sequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a subline within the Lactobacillus casei cluster. The minimal inhibitory concentration of CWBI-B623 for bile salts was higher than 1% and the survival rates to acidity with pH 3.0 and pH 2.5 were 26 and 1.2%, respectively. CWBI-B623 is an Lactobacillus casei species based on its physiological and biochemical properties and it could be a good candidate for probiotic formulations.

Key words: Lactic acid bacteria, Lactobacillus, probiotic, biodiversity.