African Journal of Parasitology Research ISSN 2343-6549 Vol. 4 (9), pp. 249-256, November, 2017. © International Scholars Journals

Full Length Research Paper

Propagation in culture of Plasmodium falciparum gametocytes clinical isolates and antiplasmodial activity of two medicinal plant extracts on parasitic growth

Beourou S1*,Okou OC2, Tuo K1, Kamenan KCD2, Gnondjui AA1, Kouakou L1, Attemene DSD3,   Penali L1, Ronan Jambou1,5, Djaman AJ1,4,6

1Laboratory of Malariology, Department of Parasitology and Mycology, Institut Pasteur of (Côte d’Ivoire),01 PO Box 490 Abidjan 01.

2Biochemistry-Microbiology Pedagogy Unit, University of Jean Lorougnon Guédé of Daloa, PO Box 150 Daloa

3Laboratory of Nutrition and Pharmacology, Faculty of Biosciences, University of Felix

Houphouet Boigny,Côte d’Ivoire. 22 PO Box 582 Abidjan 22 (Côte d’Ivoire).

4Laboratory of Biochemistry and Pharmacodynamy Faculty of Biosciences, University of Felix HouphouetBoigny, Côte d’Ivoire.22 PO Box 582 Abidjan 22 (Côte d’Ivoire).

5Institut Pasteur de Paris, Department of Parasites and Insect Vectors

6Laboratory of Fundamental Biochemistry, Institut Pasteur of Côte d’Ivoire, 01 PO Box 490 Abidjan 01.

*Corresponding author E-mail: be_sylvain@yahoo.fr

Accepted 27 September, 2017

Abstract

Gametocytes were produced from the asexual forms of clinical isolates cultured at 2.45% parasitaemia and 6% hematocrit using the candle jar method. Formation of gametocytes up to maturation (stage II to stage V) and a variable number of gametocytes going up to 1.26% for about two weeks with relatively high parasitaemia were observed.For the chemo-sensitivity tests, six crude extracts of two medicinal plants were tested on the clinical isolates and the standard strain using the SYBR Green method based on DNA fluorescence.Determination of the IC50s of our extracts after reading the 96-well microplates was performed using the IVART software from WWARN.  Results showed that the aqueous extracts (Isolate 1, IC50 = 13.27 μg / mL, Isolate 2, IC50 = 13.93 μg / mL and Isolate 3, IC50= 14.63 μg / mL and standard strain K1, IC50 = 14.01 μg / mL) and Ethanolic extracts (Isolate1, IC50 = 13.43 μg / mL, K1 strain, IC50 = 10.14 μg / mL) of Entandrophragma angolense (Lokoba) were active whereas chloroquine used as standard molecule showed mixed activity on Isolates and on chloroquine-resistant strain K1 (IC50> 50 nM). Ethanolic extracts of Cocoa nucifera were only active on isolate 3.

Key words: Gametocytes, Plasmodium falciparum, hematocrit, isolates, parasitaemia, antiplasmodial.