International Journal of Enology and Viticulture ISSN 3426-7212 Vol. 4 (2), pp. 152-157, February, 2017. © International Scholars Journals

Full Length Research Paper

Isolation and random amplified polymorphic DNA (RAPD) analysis of wild yeast species from 17 different fruits

Prashant Kumar Lathar*, Arti Sharma and Isha Thakur

Department of Biotechnology, Jay Pee University of Information Technology, Waknaghat 173234, Solan (H. P), India.

*Corresponding author. E-mail: latharpk@gmail.com. Tel: +91-9736318107.

Accepted 29 April, 2011

Abstract

The purpose of this study was to isolate the wild yeast strains present on different fruits and performing random amplified polymorphic DNA (RAPD) analysis to know the genetic inter relationship between different isolated species. Seventeen different fruit namely mango, apple, banana, black grapes, sapotae, orange, plum, jamun, pear, cherry, dates, pomegranate, figs, papaya, pineapple, green grapes and raisins were used as natural sources for yeast isolation. Amplicon fingerprints for the isolated species were obtained by RAPD assay using five different primers. Among them, only three primers allowed discrimination among the 17 isolated species. Jaccard’s genetic similarity coefficient varied from 0.00 to 0.98 (as isolate S9 did not show any relatedness with the other clads).

Key words: Random amplified polymorphic DNA, polymerase chain reaction, amplicon, dendrogram, wild yeast strains.