African Journal of Botany ISSN: 3519-3824 Vol. 5 (5), pp. 128-137, August, 2017. © International Scholars Journals
Horizontal gene transfer in cowpea (Vigna unguiculata L.Walp) through genetic transformation
1Abdulrazak B Ibrahim*, 2Sani L Abdu, 3Inuwa S Usman and 4Francisco JL Aragão
1Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria,
2Department of Plant Biology, Bayero University Kano, Nigeria,
3Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria,
4Embrapa Recursos Geneticos e Biotecnologia, LEG, PqEB W5 Norte, Brasilia DF, Brazil.
*Corresponding author E-mail: firstname.lastname@example.org. Tel: +2347034589199
Accepted 22 June, 2017
Cowpea (Vigna ungiuculata L.) is a crop with wide environmental adaptability grown in areas with extreme heat and drought, as well as in humid and wet tropical areas. Cowpea represents a crucial source of protein, especially in sub-Saharan Africa, where more than 70% of the crop is produced and consumed. Conventional breeding approaches have contributed substantially to the genetic improvement of cowpea. However, non-availability of genes for resistance to pests and viruses and sexual incompatibility with sources of resistance makes the development of insect- and virus-resistant cowpea increasingly difficult. Genetic modification of cowpea with genes of agronomic importance has the potential to overcome these problems. In this review, we summarize the key aspects of cowpea transformation work carried out in research centers around the world. We also discuss the approaches employed and the obstacles militating against efficient regeneration of transgenic cowpea expressing genes of interest.
Keywords: Genetic transformation, cowpea, Agrobacterium tumefaciens, ballistics electroporation.