International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 6 (4), pp. 767-774, April, 2017. © International Scholars Journals

Full Length Research Paper

Effect of probiotic fermented soy milk and gamma radiation on nitrosourea-induced mammary carcinogenesis

1Omayma A Ragab*, 2El-sonbaty SM, 1Aziza SA and 1Aboelftouh AE

1Biochemistry department, Faculty of Veterinary Medicine, Benha University, 2Microbiology, National Centre of Radiation Research and Technology, Atomic Energy Authority.

*Corresponding author E-mail: sawsansonbaty@yahoo.com

Accepted 02 March, 2014

Abstract

Functional food enters the concept of considering food not only as a necessity for living but also as a source of mental and physical well-being. The anti-proliferative effects of fermented soy suggested the need of low dose of gamma radiation in order to achieve an optimal therapeutic effect. This study investigated the potential chemotherapeutic effects of orally administrated fermented soy milk (FSM) with low dose of gamma radiation on mammary carcinogenesis induced by exposure to N-methyl-N-nitrosourea (MNU). There was significant immunomodulatory effect of FSM on the reduction of WBC count caused by gamma radiation or MNU. Also FSM showed ameliorative effect on GPx, SOD and CAT enzyme activities and on lipid peroxidation and TNF-α level compared to rats exposed to radiation or MNU. FSM and irradiation markedly elevated GSH content. Combined treatment of FSM and radiation ameliorated MNU effect on cell cycle phases Go/1,S,G2/M. pretreatment with FSM and gamma radiation markedly conclusion, Consumption of FSM synergizes with exposure to low doses of gamma radiation which was found to have a protective and ameliorative effect from carcinogenesis and oxidative stress induced by MNU.

Keywords: Fermented soy, N-methyl-N-nitrosourea, mammary gland, cell cycle, TNF- α, gamma radiation, antioxidant state.