International Journal of Vaccines and Immunity

International Journal of Vaccines and Immunity Vol. 2 (2), pp. 080-083, February, 2015.© International Scholars Journals

Full Length Research Paper

Management of disease using Escherichia coli antigens through vaccination

Swami Reddy and Potti Rao*

1,2Department of Virology, Faculty of Biological Sciences, University of Delhi, Delhi, India.

E-mail: rao11@yahoo.co.in.

Accepted 11 January, 2015

Abstract

A major challenge for health care in 21st century is the increase in level of resistance to pathogens and effective vaccines to prevent the often life threatening infections are urgently required. Pathogen has been investigated for their potential use either as therapeutic or prophylactic vaccines, in order to identify new antigens of diagnostic and vaccine potential. The present investigation was done with five different type of antigens produced from single pathogen. It also identifies the immune complex and DNA antigen as most suitable for vaccine development against pathogens. Antigens may vary within the host during the course of infection or antigenic types of parasites in the population. Antigenic variation is an important mechanism used by pathogenic microorganism for escaping and neutralizing activities of the antibody. Immune complexes and DNA antigen serve as vaccines, and it can be used to develop multi antigen. Furthermore, it is also easy to produce in a large scale. Vaccination is still the best way for prevention of bacterial diseases. The conditions for the preparation of antigens of intact natural composition and conformation from Escherichia coli (whole cell and heat killed), was determined using Swiss albino mice (Balb/C) as experimental species. A novel approach for vaccine design and production is discussed.

Key Words: Immune complex, vaccine, delayed type hypersensitivity, Escherichia coli.