International Journal of Otolaryngology and Ophthalmology

International Journal of Otolaryngology and Ophthalmology ISSN 7329-1943 Vol. 2 (3), pp. 028-034, March, 2016. ©International Scholars Journals

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Full Length Research Paper


A study of the significance of phenotypic and genotypic epidemiological surveillance of predominant O12 serotype clones

 

Sfar Jegham1, Abdallah Nouira1, Jegham Mzali2

 

1Laboratoire Microorganismes et Biomolécules Actives, Faculté de Sciences de Tunis, 2092 Tunis, Tunisia. 2Laboratoire de Bactériologie, Hôpital la Rabta, Tunis, Tunisia.

 

Accepted 23 September,  2015 

 

Abstract

 

Ninety four clinical isolates of Pseudomonas aeruginosa cultured from patients admitted to the Intensive Care Unit (ICU) (N= 37) and Otolaryngology (ORL) (N= 57) during one year (2001-2002) at the Rabta hospital (Tunisia) have been investigated by using serotyping, pyocintyping, drug susceptibility, M13-PCR and PFGE typing. Result shown that most of the isolates at the ICU belonged to serotype O:12 (11/37) that showed high resistance to commonly used antimicrobials ( -lactamins, aminosids, and quinolone) and a predominance for pyocinotype P10. Despite the frequent occurrence of identical serotypes and pyocinotypes, most of the isolates represent unique RAPD-M13 genotype (88/94). PFGE typing detected three distinct clusters amongst the O12 isolates, suggesting a clonal relatedness among multiresistant O12 isolates. This study illustrates the importance of phenotypic and genotypic epidemiological surveillance of predominant O12 serotype clones in such service in local hospital.


Key words: Pseudomonas aeruginosa, epidemiology, clonal diffusion, multidrug resistance.