International Journal of Microbiology Research and Reviews

International Journal of Microbiology Research and Reviews ISSN 2329-9800 Vol. 6 (4), pp. 213-223, April, 2017. © International Scholars Journals

Full Length Research Paper

Phenotypic characterization of nosocomial bacterial species on contact surfaces of veterinary clinics and hospitals in Abuja, Nigeria

Mailafia S, Olabode HOK and Akaefe I

Faculty of Veterinary Medicine, Department of Veterinary Microbiology, University of Abuja, Nigeria.

*Corresponding author E-mail: +234(080)32922883

Accepted 16 April, 2017


Nosocomial bacterial infections are responsible for a variety of diseases in man and animals. 150 Samples were conveniently collected from contact surfaces of Veterinary Clinics and Hospital and analyzed using culture, microscopy, biochemical and in-vitro antibiotic testing. The results showed  Bacillus species and Escherichia coli had  highest prevalence of 14.7% each, followed by Staphylococcus aureus (13.5%), Streptococcus uberis and Salmonella enterica (12.0% each), Listeria monocytogenes (10.7%), Corynebacterium diphtheria and Proteus vulgaris (5.4 %) each. Micrococcus luteus, Shigella, Klebsiella, and Yersinia enterocolitica  (2.7%). On premises, hospital premises ‘A’ had prevalence of 29.3% while hospital premise “B” had 17.3%.  Contact surfaces showed prevalence of 7.3% in surgical tables to 2.7% in surgical equipment. Bacterial counts revealed that 6.5 x106 and 1.0 x 104. Chi-square showed Pearson CM-square of X2=2.529   (df2).  P <0.05.  Antibiotic resistance was Amoxicillin (17%), Trimethoprim (17%), Peflacin (33%) and Cefalexin (25%). Susceptibility was Gentamycin (67%), Ciprofloxacin (88%), Ofloxacin (58%), Streptomycin (50%), Ampicillin (67%) and Nalidixic acid (33%). Minimum inhibitory concentrations were 0.125, 0.1, 0.115, 0.10, 0.125, and 0.625 µg/ml). Questionnaire survey revealed that 36% respondents had no knowledge of nosocomial infection while 62% failed to sanitize their hands. Veterinary Clinics and Hospitals in Abuja are thus contaminated with nosocomial bacteria which are resistant to antibiotics. Awareness sensitization and hygienic measures are necessary in Nigerian veterinary clinics.

Keywords: Characterization, clinics, contact surfaces, nosocomial, phenotypic, veterinary clinics.




*Corresponding author E-mail:

Tel.: +234(080)32922883