International Journal of Microbiology Research and Reviews

International Journal of Microbiology Research and Reviews ISSN 2329-9800 Vol. 6 (6), pp. 357-363, June, 2017. © International Scholars Journals

Full Length Research Paper

Monitoring the persistence of a genetically modified strain of the diazotroph Azorhizobium caulinodans in the rhizosphere of wheat (Triticum aestivum L.)

1Slaski JJ*, 1Archambault DJ+,1Vidmar JJ, 2Bécquer CJ, 1Anyia AO

1Alberta Innovates–Technology Futures, Bioresource Technologies, Vegreville, Alberta, Canada, T9C 1T4, 1+Present address: Laurentian University, 935 Ramsey Lake Road, Sudbury, ON, Canada, P3E 2C6, 2Ministerio de la Agricultura, Instituto de Investigaciones de Pastos y Forrajes, Estación Experimental Sancti Spíritus, Cuba. Apdo 2255, Z.P. 1, Sancti Spíritus, Cuba.

*Corresponding author E-mail: jan.slaski@albertainnovates.ca 

Accepted 14, December, 2016

Abstract

A protocol was developed to monitor persistence and spread of a genetically modified strain of the growth promoting diazotroph Azorhizobium caulinodans used to inoculate field grown wheat. The protocol was used to identify an endogluconase(egl) overproducing strain of A. caulinodansre-isolated from inoculated soils. A detection limit of 2500 cfu/g and 108–1010CFU/g of soil was determined using the BIOLOG® fingerprinting method and PCR technique, respectively. Since none of the tested samples were positive for the bacteria or the Egl 1 gene construct, it was concluded that A. caulinodans ORS 571 pGV910-C1 did not persist or spread in any of the tested field locations. This result may be due to low soil temperatures and competition of indigenous microorganisms: environmental factors that were not favorable for the diazotroph to thrive in the test locations. The application of different inoculation methodologies, as well as the study of other rhizobial genera for the inoculation of wheat in further experiments, is strongly recommended.

Keywords: ORS 571, detection, diazotroph, endogluconase, wheat.