International Journal of Microbiology Research and Reviews

International Journal of Microbiology Research and Reviews ISSN 2329-9800 Vol. 6 (2), pp. 197-204, February, 2017. © International Scholars Journals

Full Length Research Paper

Bacterial isolates from poultry litters and their antimicrobial susceptibility patterns in Gondar, Northwest Ethiopia

1Abebe Eyasu, 2Feleke Moges and3Agersew Alemu*

1Dessie regional laboratory, Amhara regional state, North East  Ethiopia,

2Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia,

3Department of Medical Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

*Corresponding author E-mail: dagimagersew@gmail.com 

Accepted 12 November, 2014

Abstract

Poultry wastes or litters are generally added to the soil as a fertilizer. The final step in the poultry farming management strategy entails a big risk for the environment due to the nutrients and microorganisms contained in high concentrations in these waste materials. The objective of this study was to isolate bacteria from poultry wastes and test their antimicrobial susceptibility patterns. A cross sectional study was conducted. Forty four samples were analyzed from five poultry farms, in Gondar town from February, 2012 to March, 2012. A total of 52 bacterial pathogens were isolated from 44 samples of poultry wastes.   Gram-negative bacteria were more prevalent 35 (67.3%) than Gram-positive bacteria 13(32.5%). Isolated organisms having public health importance include E.coli, S.aureus, Proteus vulgaris, Klebsiella pneumonia, Serratia spp., Enterobacter cloacae, Enterobacter aerogenes, Coagulase negative staphylococci (CNS), Citrobacter diversus and other Citrobacter spp,. The most commonly isolated bacteria were E. coli 24 (46%) followed by S. aureus 10 (19%), CNS 7(13.5 %), Enterobacter aerogenes 3(5.8), Enterobacter cloacae 2 (4%), Serratia spp., 2(4%) and others 4(7.7%). All bacterial isolates demonstrate multi-drug resistant for tested antimicrobials.  High antimicrobial resistant was observed for ampicillin (94%), penicillin (92%), tetracycline (64%), erythromycin 56%), whereas, Low antimicrobial resistance was seen for Kanamycine (2%), Ciprofloxacilin (4%), and chloramphinicol (11%). no resistant was observed for Vancomycin and methicillin. Medically important multidrug resistance species were isolated from poultry litters and some of the bacterial isolates are potentially pathogenic for humans and animals. The present study recommends proper information dissemination to farmers and poultry feeds producers about the public health importance of proper poultry litter disposal.

Keywords: Multidrug resistance, poultry litter, susceptibility.