International Journal of Horticulture and Floriculture
International Journal of Horticulture and Floriculture ISSN 2167-0455 Vol. 5 (1), pp. 247-251, January, 2017. © International Scholars Journals
Full Length Research Paper
Response of cucurbitaceous rootstocks and bitter gourd scions to root knot nematode Meloidogyne incognita Kofoid and White
N.A.Tamilselvi1*, L.Pugalendhi2 and M.Sivakumar3
1Department of Vegetable Crops, 3Department of Nematology,Tamil Nadu Agricultural University, Coimbatore- 641 003.
2Tapioca and Castor Research Station, Yethapur, Salem-636 119.
Received 21 July, 2016; Revised 08 October, 2016; Accepted 19 November, 2016 and Published 02 January, 2017
Root knot nematodes are responsible for severe crop loss in bitter gourd to the tune of 38-48.2%. Pot culture experiments were conducted under glasshouse conditions to study the effect of root knot nematode, Meloidogyne incognita Kofoid and Whitein ten cucurbitaceous species and to identify resistant rootstocks for grafting in bitter gourd. Second stage juveniles of M. incognita were inoculated at 2J2 per cc soilinto the rhizosphere of 21 days old seedlings. Results revealed that, three genotypes viz.,Kumatikai (Citruluscolocynthis), African horned cucumber (Cucumismetuliferus) andpumpkin (Cucurbita moschata) showed resistant reaction followed by two rootstocks viz.,Sponge gourd (Luffa cylindrica) andmithipakal (Momordicacharantia var. muricata) which are moderately resistant. The biochemical activitiesviz., Polyphenol oxidase (PPO), peroxidase (PO)and phenylalanine ammonia lyase (PAL)showed superiority in the aforementioned species. These rootstocks are further used for grafting with bitter gourd scions.
Keywords: Root knot nematode, Meloidogyne incognita, resistance,bitter gourd, Biochemical parameters, Polyphenol oxidase, Peroxidase, Phenylalanine ammonia lyase.
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