African Journal of Parasitology Research

African Journal of Parasitology Research ISSN: 2343-6549 Vol. 4 (5), pp. 219-226, May, 2017. © International Scholars Journals

Full Length Research Paper

Comparative study between  the Baermann conventional and a simplified Baermann devices for the diagnosis of Strongyloides stercoralis

1,2Kiki-Barro PCM*, 4Aka Hepkangjin J, 2,4Kassi FK, 1,2Henriette Vanga-Bosson H, 2Konaté A, 2Angora EK, 2Akoua Valérie Bedia-Tanoh AV, 2Djohan V, 1Kamagaté T, 2Sébastien Miezan, 4N’guessan NA, 1,2William Yavo W and 2,3Menan EIH

1Parasitology and Mycology Department, National Institute of Public Health, BPV 47, Abidjan, Côte d’Ivoire, 2Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’Ivoire, 3Parasitology and Mycology Laboratory of Diagnosis and Research Centre on AIDS and Other Infectious Diseases, 4Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’Ivoire.

E-mail: kikipcm@yahoo.fr. Phone: +22507584947

Accepted 18 May, 2017

Abstract

The-conventional Baermann device components are a constraint during its use for field investigations. A simplified version is proposed in order to assess the agreement of the measurements and the cost between both devices. 101 stool samples from HIV positive subjects were examined using both devices. The positivity rate was 0.9-% (1/101). The stool tested positive was subjected to 7 successive dilutions by means of a  Strongyloides stercoralis larvae free stool. Each dilution, considered as a sample, was used to compare the accuracy agreement of both devices. The Student t test did not show any significant variation in the number of the extracted larvae per gram of stool (p=0.227). The correlation coefficient (0.9991) close to 1 indicates a high reliability between both devices. The measurement of the larvae (per gram of stool)  using both devices agree as shown by the Bland Altman analysis, with a positive bias. Bias = 0.5 ± 1.069; 95% CI (- 0.39 to 2.59). The cost of the analysis was estimated at $ US 30.31 for the conventional device versus $US 2.23 for the simplified one. The latter is an alternative for the strongyloidiasis diagnosis during large-scale investigations in countries with limited resources.

Keywords: Strongyloidiasis, diagnosis, Baermann, devices, Comparison, agreement, Abidjan, Côte d’Ivoire.

 


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