African Journal of Internal Medicine

African Journal of Internal Medicine ISSN 2326-7283 Vol. 5 (4), pp. 406-411, April, 2017. © International Scholars Journals

Full Length Research Paper

In vitro activity of antibacterial drugs upon microorganisms from patients with chronic otitis media

1Özalkan Özkan*, 2Hakan Uslu, 2Dilek Vural Keleş, 1Fatih Bingöl, 1Murat Yaşar, 1Harun Üçüncü*

1Ataturk University, Faculty of Medicine, Deparment of Otorhinolaryngology, Erzurum, Turkey, 2Ataturk University, Faculty of Medicine, Deparment of Microbiology, Erzurum, Turkey.

*Corresponding author E-mail: ozalkanozkan@gmail.com

Tel:04425111530, Fax:04422361301

Accepted 12 May, 2014

Abstract

This study was conducted to generate microorganisms isolated from cultures of ears taken from the patients diagnosed to have chronic otitis media and determine and compare the in vitro effects of different antibiotics on these microorganisms. Between June 2009 and October 2010, 127 ear cultures were taken from 100 patients who applied to the Department of Otolaryngology of Ataturk University Faculty of Medicine Hospital diagnosed to have chronic otitis media in the otolaryngological investigation. Antimicrobial susceptibility of isolated strains was determined by the disc diffusion method according to the CLSI criteria. From the cultures investigated, 277 (6 ferment and 16 fungus) microorganisms were generated. Among the isolated pathogen microorganisms, pseudomonas spp. (24,91%), staphylococcus spp (13,00%), proteus spp. (5,05%) were the three most frequently isolated pathogen agents. 4 Peptococcus spp. (1,44%) and 3 Peptostreptecoccus spp. (1,08%) multiplied and it was observed that there was 2,52% anaerobic reproduction among all microorganisms. It was also seen that Pseudomonas spp. strains were more susceptible to gentamicin (97,1%), imipenem (94,2%), amikacin (84,1%) and ciprofloxacine (78,3%). In the study, vancomycine, teicoplanin, telithromycin and linozolidin were found to be the most effective antibiotics for all staphylococcus. It was seen that proteus spp. strains were more susceptible to gentamicin (92, 9%), ciprofloxacin (%92, 9) and piperacillin (92, 9%). In the antimicrobial treatment required to reach effective outcomes in the treatment of patients with chronic otitis media, it is important to monitor the frequency of effective microorganisms and their rates of resistance to antimicrobial agents.

Keywords: Chronic otitis, antibiogram, microorganism, in vitro, antibacterial.