African Journal of Biotechnology Research

African Journal of Biotechnology Research ISSN 9316-2194 Vol. 4 (4), pp. 179-188, April, 2016. © International Scholars Journals

Full Length Research Paper

Study of the indol 3- acetic acid (IAA) induced inhibition of growth and sporulation of Fusarium oxysporum F. sp. lentis as the causal organism of wilt disease of lentil

Salman Modi Singh Dhoni

Department of Botany, S.P.D. College, Garhwa, PIN 822114, Jharkhand State, India.

E-mail: salama_modi@yahoo.com

Accepted 16 March, 2016

Abstract

The wilt disease in lentil crop is a wide spread and recurrent phenomenon. Control of the disease by current technique is not sufficient. To investigate the suitable control measures by bio-molecules is highly desired. Bio-molecules are non-polluting self metabolized and biologically regulated compounds have well defined physiological action without interfering with other actions in the host plant. Inhibitory role of growth hormones and growth regulators have been indicated by many fungi. Inhibitory action of indol 3- acetic acid (IAA) on mycelial growth and sporulation of Fusarium oxysporum Schlecht. F. sp. lentis (Vasudeva and Srinivasan) as the causal organism of wilt disease of lentil (Lens esculenta Moench.) under in vitro culture have been carried out. The result indicates that mycelia growth was gradually inhibited with increase in concentration of IAA from 0.1 to 1.0 ppm. Further increase in concentration from 1.0 to 10.0 ppm, however, showed increase in growth. Sporulation of macroconidia and microconidia also reduced in number with increase in concentration from 0.1 to 1.0 ppm, though the number remained more than that of the control. Further increase in concentration from 1.0 to 10.0 ppm sporulation of both the spores increased. Sporulation of chlamydospores increased with increase in concentration from 0.1 to 1.0, thereafter inhibition of sporulation was recorded with increase in concentration from 1.0 to 10.0 ppm of IAA. This result indicated the inhibitory action of IAA for mycelia growth but sporulation was not completely inhibited.

Key words: Wilt disease, lentil wilt, Fusarium oxysporum f. sp. lentis, Indol 3- acetic acid