African Journal of Biotechnology Research

African Journal of Biotechnology Research ISSN 9316-2194 Vol. 4 (8), pp. 206-211, August, 2016. © International Scholars Journals

Full Length Research Paper

Production of efficient protocol for regenerating plantlets from the undifferentiated callus tissues

Deepek R. Chandra1*, Asha B. Subbulakshmi2 and R. P. Sreedharan2

1Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India,

2Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North Eastern Hill University, Shillong - India.

E-mail: deepek.drm2@gmail.com

Accepted 15 June, 2016

Abstract 

Mantisia wengeri Fischer is a rare and endemic zingiber of North-East India which has been traditionally used for curing bone fracture and gastro-intestinal ailments. However due to the extreme rarity of the germplasm, studies related to genetic diversity, phytochemical properties etc. in this plant has not been feasible. The study describes the development of high efficiency regeneration protocol for obtaining large- scale diverse plantlets from rhizomatous callus tissues in Murashige and Skoog medium incorporated with various concentration of growth regulators N6-benzyladenine (BA), α-Napthalene acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and sucrose at elevated concentrations. Bud forming capacity (BFC) to a maximum of 33.5±3.7 was recorded from the organogenic callus tissues of M. wengeri within 8 - 10 weeks in MS medium supplemented with a combination of 10µM of BA and 40µM NAA at 10% sucrose. The callogenic plantlets are been transferred to the field for studying morphological and genetic variations.

Key words: - Conservation, regeneration, genetic variation, rhizomatous, callus

 

 

African Journal of Biotechnology Research ISSN 9316-2194 Vol. 4 (8), pp. 206-211, August, 2016. Available

online at www.internationalscholarsjournals.org © International Scholars Journals 

Author(s) retain the copyright of this article.

 

 

Full Length Research Paper

 

Production of efficient protocol for regenerating plantlets from the undifferentiated callus tissues

 

Deepek R. Chandra1*, Asha B. Subbulakshmi2 and R. P. Sreedharan2

 

Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India, 2Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North Eastern Hill University, Shillong - 793

022, India

 

Accepted 15 June, 2016

 

Mantisia wengeri Fischer is a rare and endemic zingiber of North-East India which has been traditionally used for curing bone fracture and gastro-intestinal ailments. However due to the extreme rarity of the germplasm, studies related to genetic diversity, phytochemical properties etc. in this plant has not been feasible. The study describes the development of high efficiency regeneration protocol for obtaining large- scale diverse plantlets from rhizomatous callus tissues in Murashige and Skoog medium incorporated with various concentration of growth regulators N6-benzyladenine (BA), α-Napthalene acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and sucrose at elevated concentrations. Bud forming capacity (BFC) to a maximum of 33.5±3.7 was recorded from the organogenic callus tissues of M. wengeri within 8 - 10 weeks in MS medium supplemented with a combination of 10µM of BA and 40µM NAA at 10% sucrose. The callogenic plantlets are been transferred to the field for studying morphological and genetic variations.

 

Key words: - Conservation, regeneration, genetic variation, rhizomatous, callus