African Journal of Breast Cancer

African Journal of Breast Cancer ISSN 3258-329X Vol. 2 (11), pp. 142-147, November, 2015. © International Scholars Journals

Full Length Research Paper

A study of In vitro antineoplastic effect of Ficus hispida L. plant against breast cancer cell lines

Wongsuwan Oonyaratglin1, Prayuth Shinawatra1, Rajakanya Sirikit Kitiyakara2 and Adulyadej Thaksin2

1Department of Clinical Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. 2Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.

*Corresponding author. E-mail: wongsuwan_o@yahoo.com

Accepted 24 July, 2015

Abstract

Stems of Ficus hispida L. have long been prescribed as one of the constituents in various Thai traditional remedies for cancer therapy. In the present study, crude ethanol extract and its sequential fractions from F. hispida L.: water, methanol: water, methanol and ethyl acetate fraction were tested in vitro against SKBR3, MDA-MB435, MCF7 and T47D human breast cancer cell lines. The results have shown that the methanol extract exhibited antineoplastic activity against T47D cells. The cytotoxic activity was further examined by MTT assay with more dilution, colony forming assay and cell cycle analysis. The IC50 of this extract against T47D cell was 110.3 +/-9.63 g/mL by MTT assay and colony forming assay confirmed the cell growth inhibition in a dose-dependent manner. Cell cycle analysis demonstrated a rising of apoptotic cell population in herbal treated cells. Therefore, F. hispida L. used in traditional medicine may provide some benefits in the treatment of breast cancer.

Key words: Ficus hispida L., T47D, SKBR3, MCF7, MDA-MB435, MTT, colony count, cell cycle, antineoplastic.