African Journal of Biology

African Journal of Biology ISSN 2167-0413 Vol. 3 (7), pp. 258-264, July, 2016. © International Scholars Journals

Full Length Research Paper

Investigation of drug susceptibility in S. aureus isolated from burn patients in Missan city

*Abd al-Ilah Salbi, Usama S. Kahraman and Faisal V. Ashkouri

Department of Biology, College of Science, Missan University, Iraq.

E-mail: prof.salbi3444@yahoo.com 

Accepted 28 June 2016

Abstract 

Twenty one isolates of Staphylococcus aureus collected from Alsader a hospital – Missan then were analyzed between (October and December in 2013). The samples taken in order to determine the bacterial profile and antibiotic susceptibility. Isolates of S. aureus were tested against 8 differrent antibiotics and crude of methanol extracts of Capsicum annuum L.var. (Solanaceae) by a disk diffusion method. 100% of the isolates were resistant to the Penicillin, 95% resistant to Lincomycin , 90.4% Tetracyclin. 90.4% Rifampin, 80.9% resistant to Nitrofuraantion and 57.1% resistant to Bacteriacin were found to be the least effective antibiotics while 9.5% resistant to Amicacine and 38.09% resistant to Novobiocine where more effective antibiotics against (MRSA) S. aureus. The minimum inhibitory concentration (MIC) (mg/ml) of the alcoholic were also determined. Also the antimicrobial suscepitlity of isolates where tested against methanol extract of C. annuum with different concentration (500, 250, 125, 62.5 µฤก/ml) detected by agar well diffusion methods. The alcoholic extracts proved to be more effective than aqueoous extracts antimicrobials against (MRSA) S. aureus. Finally cytotoxicity evaluating towards human RBC, the results revealed these crud extracts of C. annuum have not any cytotoxicity in all coocentrations. The good antimicrobial potency of the methanol extract of C. annuum plants indicates the tretment of (MRSA) as an alternative to the costly antibiotics.

Key words: Antibiotic, minimum inhibitory concentration (MIC), MRSA (Staphylococcus aureus), Capsicum annuum L., cytotoxicity.