African Journal of Agriculture

African Journal of Agriculture ISSN 2375-1134 Vol. 3 (11), pp. 247-253, December, 2016. © International Scholars Journals

Full Length Research Paper

Enzyme activities and expression of genes in plant tolerance to low temperatures in dry maize seed

Sérgio Naymer Amaral*, Paulo C. Fernando, Tarsila Vieira Carlos and Jpse Braga Amado

Department of Agriculture, Seeds Laboratory, Federal University of Lavras (Universidade Federal de Lavras – UFLA), University Campus – P.O. Box 3037, ZIP code 37200-000, Lavras MG, Brazil.

E-mail: neymar04@ymail.com

Accepted 15 November, 2016

Abstract

The study was conducted on dry maize seed and seed subjected to sub optimal germination temperature. Four lines of maize seeds were used, two were classified as susceptible to low temperature (44 and 54) and two tolerant to low temperature (64 and 91°C). Water content and germination tests were performed at different temperatures (10 and 25°C) to evaluate the physical and physiological quality of maize seeds. For molecular study, activity of heat-resistant proteins, esterase, superoxide dismutase, catalase and α-amylase enzymes were assessed. Study of AOX1, ZmMPK5, SOD, APX, SAD, ENR and LEA genes with real time polymerase chain reaction (PCR) were carried out. AOX, SAD and LEA genes that are related as tolerant to abiotic stress showed higher expression in line 64, which was classified as tolerant to low germination temperature. The ZmMPK5 gene is associated with increased production of abscisic acid and expressed more in line 54, which was considered as susceptible to low germination temperature.

Key words: Zea mays, seed vigor, abiotic stress.