African Journal of Poultry Farming

 African Journal of Poultry Farming ISSN 2375-0863 Vol. 5 (3), pp. 177-184, March, 2017. © International Scholars Journals

Full Length Research Paper

Safety and efficacy studies of Newcastle Disease vaccines in very young African local ecotype chicks and in commercial pullets

1Domingue G*, 1Peters, A 2Muhairwa AP, 2Chiwanga GH, 2Msoffe PL, 3Jaglarz A, 4Wachira J, 4Musau AM, 4Kyangu NK and 1Thevasagayam S

1GALVmed (Global Alliance for Veterinary Medicines), Doherty Building, Pentlands Science Park, Edinburgh.EH26 0PZ, Scotland,

2SUA (Sokoine University of Agriculture), Department of Veterinary Medicine and Public Health, Faculty of Veterinary Medicine, Sokoine, P.O. Box 3021, Chuo Kikuu Morogoro, Tanzania,

3MRI (Moredun Research Institute), Pentlands Science Park, Edinburgh, EH26 0PZ. Scotland, 4KEVEVAPI (Kenya Veterinary Vaccine Production Institute), Vaccine Production Building, Veterinary Investigation Labs, Nairobi, Kenya.


Received 31 October, 2016; Revised 04 December, 2016; Accepted 13 December, 2016 and Published 14 March, 2017


Two Good Clinical Practice studies are described. Firstly, the safety and efficacy of live, attenuated LaSota and l-2 Newcastle Disease (ND) vaccines and inactivated, adjuvanted ITA-NEW ND vaccine were evaluated in eight-day old local ecotype chicks. For all vaccines safety and efficacy were satisfactory and serological titres exceeded the putative protective level of >23 before 14 days post-vaccination. The vaccinated groups displayed no significant differences.  The data suggest that these vaccines are effective in very young village poultry. Secondly, in 35 day-old ISA-Brown pullets, MSD a 10x field dose formulation of Clone 30 vaccine, was compared to I-2 after a heat-stress test approximating to local conditions of delivery and use (24h, 32.3oC, in the dark). By 14 days post-vaccination, the heated MSD vaccine and heated I-2 titres exceeded 23 but the response of the heated MSD group was significantly higher than the heated I-2 group. Non-heated MSD induced a very rapid and higher response than those induced by the heated vaccines, as by 7 days post-vaccination, a 23 titre was reached and exceeded (GMT 4.0). The 10x normal field dose approach to conferring thermotolerance to live vaccines appears to be a simple, cheap and pragmatic method for use in hot climates.

Keywords: Newcastle Disease vaccines, agricultural development, thermotolerance, LaSota, I-2, ITA-NEW, Clone 30.