Advances in Agrotechnology

Advances in Agrotechnology ISSN: 9082-1831 Vol. 1 (7), pp. 068-072, December, 2017. © International Scholars Journals

Full Length Research Paper

Effects of different combination concentrations of BAP and NAA on types of explants and its regeneration

*Lim D. Yoong, Karpal Tan Kwame and Loganathan  Chaw

Department of Agrotechnology, Faculty of Agrotechnology and Food Sciences, Universiti Malaysia Terengganu,

Mengabang Telipot, 21030 Kuala Terengganu, Malaysia.


Accepted 12 November, 2017


We present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h photoperiod at light intensity of 22.85 µmol /m2 /s) for 8 weeks. Results showed that petiole explants cultured at combination of 1.0 mg/L BAP and 0.2 mg/L NAA gave highest number of shoot produced per explants (4.33 ± 0.33) and a maximum callus fresh weight gains (3.2 ± 1.9 g) as compared to other treatments. The first shoot was observed from petiole cultured at all treatments in 4 weeks culture except for 0.1 mg/L BAP + 0.5 mg/L NAA treatment. For root induction, treatment at 0.1 mg/L NAA produced the highest number of roots produced per shoot (9.8 ± 3.1) compared to other treatments. Regeneration comparison between petiole, young leaf and stem segments showed that petiole is the most suitable explant for an efficient plant regeneration system of O. stamineus.

Key words: Orthosiphon stamineus, plant regeneration, shoot regeneration, benzylaminopurine (BAP), naphthalene acetic acid (NAA).